{"id":343,"date":"2015-04-03T16:03:47","date_gmt":"2015-04-04T00:03:47","guid":{"rendered":"http:\/\/probemonkey.com\/?page_id=343"},"modified":"2018-10-23T15:08:28","modified_gmt":"2018-10-23T23:08:28","slug":"structures","status":"publish","type":"page","link":"https:\/\/probemonkey.com\/?page_id=343","title":{"rendered":"Structures"},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"color: #000000;\">The G18V variant of human \u03b3S-crystallin (\u03b3S-G18V) is implicated in childhood-onset cataract. We have investigated \u03b3S-G18V and its symmetry-related variant \u03b3S-G106V, located at the analogous position on the C-terminal domain, and found that these two superficially similar mutations affect stability and solubility quite differently. The solution NMR structures of the wild-type protein (\u03b3S-WT) and \u03b3S-G18V and their interactions with \u03b1B-crystallin reveal that the aggregation mechanism of \u03b3S-G18V is related to altered intermolecular interactions of a native-like monomer rather than large-scale misfolding or remodeling of the protein structure. Our results for this system are consistent with a cataractogenesis model where the aggregation of \u03b3S-G18V results from depletion of the lens\u2019s finite pool of \u03b1-crystallin. Ongoing studies include the investigation of other clinically relevant mutations, as well as those mimicking the effects of age-related cataract.<\/span><\/p>\n<h3 style=\"text-align: right;\"><span style=\"color: #000000;\"><a href=\"https:\/\/probemonkey.com\/wp-content\/uploads\/2015\/04\/WT_gS.png\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-578 size-medium alignright\" src=\"https:\/\/probemonkey.com\/wp-content\/uploads\/2015\/04\/WT_gS-300x213.png\" alt=\"\" width=\"300\" height=\"213\" srcset=\"https:\/\/probemonkey.com\/wp-content\/uploads\/2015\/04\/WT_gS-300x213.png 300w, https:\/\/probemonkey.com\/wp-content\/uploads\/2015\/04\/WT_gS.png 733w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/span><\/h3>\n<h3><span style=\"color: #000000;\">Wild Type Human Gamma-S Crystallin<\/span><\/h3>\n<h3 style=\"text-align: right;\"><\/h3>\n<h3><\/h3>\n<p><span style=\"color: #00ff00;\"><a style=\"color: #00ff00;\" href=\"http:\/\/link.springer.com\/article\/10.1007%2Fs12104-011-9326-1\">Publication<\/a> \u00a0 \u00a0\u00a0 \u00a0\u00a0\u00a0 <a style=\"color: #00ff00;\" href=\"http:\/\/www.rcsb.org\/pdb\/explore\/explore.do?structureId=2M3T\">PDB<\/a>\u00a0\u00a0\u00a0\u00a0 \u00a0 \u00a0\u00a0 <\/span><span style=\"color: #333333;\"><span style=\"color: #00ff00;\"><a style=\"color: #00ff00;\" href=\"http:\/\/www.bmrb.wisc.edu\/data_library\/summary\/index.php?bmrbId=17576\">BMRB<\/a>\u00a0\u00a0\u00a0\u00a0<\/span><br \/>\n<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"color: #000000;\">\u00a0<\/span><\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000000;\"><a href=\"https:\/\/probemonkey.com\/wp-content\/uploads\/2015\/04\/G18V_gS.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-581 size-medium\" src=\"https:\/\/probemonkey.com\/wp-content\/uploads\/2015\/04\/G18V_gS-300x213.png\" alt=\"\" width=\"300\" height=\"213\" srcset=\"https:\/\/probemonkey.com\/wp-content\/uploads\/2015\/04\/G18V_gS-300x213.png 300w, https:\/\/probemonkey.com\/wp-content\/uploads\/2015\/04\/G18V_gS.png 733w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>G18V Human Gamma-S Crystallin<\/span><\/h3>\n<p><span style=\"color: #333333;\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"http:\/\/link.springer.com\/article\/10.1007%2Fs12104-011-9326-1\">Publication<\/a> \u00a0 \u00a0 \u00a0 \u00a0\u00a0 <a style=\"color: #0000ff;\" href=\"http:\/\/www.rcsb.org\/pdb\/explore\/explore.do?structureId=2M3U\">PDB<\/a>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <a style=\"color: #0000ff;\" href=\"http:\/\/www.bmrb.wisc.edu\/data_library\/summary\/index.php?bmrbId=17582\">BMRB<\/a><\/span><br \/>\n<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The G18V variant of human \u03b3S-crystallin (\u03b3S-G18V) is implicated in childhood-onset cataract. We have investigated \u03b3S-G18V and its symmetry-related variant&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"page-templates\/page-fullwidth.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-343","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/probemonkey.com\/index.php?rest_route=\/wp\/v2\/pages\/343","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/probemonkey.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/probemonkey.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/probemonkey.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/probemonkey.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=343"}],"version-history":[{"count":8,"href":"https:\/\/probemonkey.com\/index.php?rest_route=\/wp\/v2\/pages\/343\/revisions"}],"predecessor-version":[{"id":915,"href":"https:\/\/probemonkey.com\/index.php?rest_route=\/wp\/v2\/pages\/343\/revisions\/915"}],"wp:attachment":[{"href":"https:\/\/probemonkey.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=343"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}